Research

Publications

Below you can find a list of our published research.

6125 results

Cited 48 times since 2000 (2 per year) source: EuropePMC

Circulation research, Volume 87, Issue 3, 1 1 2000, Pages 248-253 Accelerated atherosclerosis by placement of a perivascular cuff and a cholesterol-rich diet in ApoE*3Leiden transgenic mice. Lardenoye JH, Delsing DJ, de Vries MR, Deckers MM, Princen HM, Havekes LM, van Hinsbergh VW, van Bockel JH, Quax PH

Intimal hyperplasia after vascular injury is usually studied in animal models with healthy, normocholesterolemic animals. Here, we assess the effect of diet-induced hypercholesterolemia on the induction of intimal hyperplasia in ApoE*3Leiden mice. A nonconstrictive polyethylene cuff was placed around the femoral artery of ApoE3*Leiden mice fed a highly cholesterol-rich diet, a mildly cholesterol-rich diet, or a chow diet for 4 weeks. Diets were continued after cuff placement until euthanization.... Abstract

Cited 32 times since 2000 (1.3 per year) source: EuropePMC

Arthritis and rheumatism, Volume 43, Issue 8, 1 1 2000, Pages 1710-1718 Cartilage degradation and invasion by rheumatoid synovial fibroblasts is inhibited by gene transfer of a cell surface-targeted plasmin inhibitor. van der Laan WH, Pap T, Ronday HK, Grimbergen JM, Huisman LG, TeKoppele JM, Breedveld FC, Gay RE, Gay S, Huizinga TW, Verheijen JH, Quax PH

Objective: Joint destruction in rheumatoid arthritis (RA) is a result of degradation and invasion of the articular cartilage by the pannus tissue. The present study was undertaken to examine the role of the plasminogen activation system in cartilage degradation and invasion by synovial fibroblasts and investigate a novel gene therapeutic approach using a cell surface-targeted plasmin inhibitor (ATF.BPTI). Methods: Adenoviral vectors were used for gene transfer. The effects of ATF.BPTI gene trans... Abstract

Cited 22 times since 2000 (0.9 per year) source: EuropePMC

The Journal of thoracic and cardiovascular surgery, Volume 120, Issue 1, 1 1 2000, Pages 55-65 Remodeling of the porcine pulmonary autograft wall in the aortic position. Schoof PH, Gittenberger-De Groot AC, De Heer E, Bruijn JA, Hazekamp MG, Huysmans HA

Objective: Dilatation and valve regurgitation are disturbing sequelae of the pulmonary root functioning at systemic pressures. We tried to characterize the histologic mode of adaptation of the neoaortic wall. Methods: We compared routine histologic studies, immunohistochemical staining, and computer-assisted morphometric analysis of aortic, pulmonary autograft, and native pulmonary wall specimens from pigs in which, as a newborn, a valveless pulmonary autograft had been implanted in the aorta. R... Abstract

Cited 17 times since 2000 (0.7 per year) source: EuropePMC

Journal of clinical microbiology, Volume 38, Issue 6, 1 1 2000, Pages 2065-2075 Monoclonal antibodies directed against conserved epitopes on the nucleocapsid protein and the major envelope glycoprotein of equine arteritis virus. Weiland E, Bolz S, Weiland F, Herbst W, Raamsman MJ, Rottier PJ, De Vries AA

We recently developed a highly effective immunization procedure for the generation of monoclonal antibodies (MAbs) directed against the porcine reproductive and respiratory syndrome virus (E. Weiland, M. Wieczorek-Krohmer, D. Kohl, K. K. Conzelmann, and F. Weiland, Vet. Microbiol. 66:171-186, 1999). The same method was used to produce a panel of 16 MAbs specific for the equine arteritis virus (EAV). Ten MAbs were directed against the EAV nucleocapsid (N) protein, and five MAbs recognized the maj... Abstract

Cited 45 times since 2000 (1.8 per year) source: EuropePMC

Herz, Volume 25, Issue 4, 1 1 2000, Pages 356-364 Arrhythmogenic right ventricular dysplasia: MRI findings. van der Wall EE, Kayser HW, Bootsma MM, de Roos A, Schalij MJ

Arrhythmogenic right ventricular dysplasia (ARVD) is a heart muscle disorder of unknown cause that is characterized pathologically by fibrofatty replacement of the right ventricular myocardium. Clinical manifestations include structural and functional malformations of the right ventricle, electrocardiographic abnormalities, and presentation with ventricular tachycardias with left bundle branch pattern or sudden death. The disease is often familial with an autosomal inheritance. In addition to ri... Abstract

Cited 1 times since 2000 (0 per year) source: EuropePMC

International journal of cardiology, Volume 73, Issue 3, 1 1 2000, Pages 209-211 Detecting hibernating myocardium: how and why? Siebelink HJ, Underwood SR, van der Wall EE, Blanksma PK

Abstract

Cited 11 times since 2000 (0.4 per year) source: EuropePMC

Critical care medicine, Volume 28, Issue 5, 1 1 2000, Pages 1599-1606 Evaluation of a new transcardiac conductance method for continuous on-line measurement of left ventricular volume. Steendijk P, Lardenoye JW, van der Velde ET, Schalij MJ, Baan J

Objective: To evaluate a new, less invasive, conductance method to measure continuous on-line left ventricular volume. End-systolic and end-diastolic volumes obtained with this transcardiac conductance method were compared with simultaneous measurements using the conventional intracardiac conductance catheter. Design: Controlled animal study. Setting: Research laboratory in a university hospital. Subjects: Six sheep. Interventions: Anesthetized sheep were instrumented and inotropic condition was... Abstract

Cited 28 times since 2000 (1.1 per year) source: EuropePMC

Annals of surgical oncology, Volume 7, Issue 4, 1 1 2000, Pages 268-275 Systemic toxicity and cytokine/acute phase protein levels in patients after isolated limb perfusion with tumor necrosis factor-alpha complicated by high leakage. Stam TC, Swaak AJ, de Vries MR, ten Hagen TL, Eggermont AM

Background: Since the introduction of high-dose tumor necrosis factor-alpha (TNFalpha) in the setting of isolated limb perfusion (ILP) in the clinic, prevention of leakage to the body of the patient is monitored with great precision for fear of TNF-mediated toxicity. That we observed remarkably little toxicity in patients with and without leakage prompted us to determine patterns of cytokines and acute phase proteins in patients with high leakage and in patients without any leakage. Methods: TNF... Abstract

Cited 3 times since 2000 (0.1 per year) source: EuropePMC

Annals of the New York Academy of Sciences, Volume 902, 1 1 2000, Pages 17-24; discussion 24-6 Tailored therapy to fit individual profiles. Genetics and coronary artery disease. Jukema JW, Kastelein JJ

In the twentieth century, coronary artery disease (CAD) was the major cause of morbidity and mortality in western societies. Genetics and environmental influences clearly contribute to CAD. Our genetic makeup contributes to not only coronary risk factors, but also determines an individual's response to environmental challenges such as diet, drugs, and tobacco. Genetic testing is likely to be more predictive of our predisposition to CAD than current conventional testing of known risk factors... Abstract

Cited 12 times since 2000 (0.5 per year) source: EuropePMC

British journal of pharmacology, Volume 129, Issue 7, 1 1 2000, Pages 1413-1422 Cardiovascular profile of the calcium sensitizer EMD 57033 in open-chest anaesthetized pigs with regionally stunned myocardium. de Zeeuw S, Trines SA, Krams R, Verdouw PD, Duncker DJ

1. Ca(2+) sensitizers enhance systolic function, but may impair relaxation in vitro; these effects may differ in stunned and normal myocardium. We therefore studied the effect of EMD 57033 on systolic and diastolic function of normal and stunned porcine myocardium in vivo. 2. Myocardial stunning by 15 min coronary occlusion and 30 min reperfusion abolished systolic shortening (SS) (baseline 13+/-1%) and decreased end-systolic elastance (E(es)) from 67+/-7 to 47+/-5 mmHg mm(-1) (both P Abstract

Cited 4 times since 2000 (0.2 per year) source: EuropePMC

The Journal of invasive cardiology, Volume 12, Issue 4, 1 1 2000, Pages 187-193 Clinical and angiographic outcome of stent implantation without predilatation using the Jostent Flex stent. Oemrawsingh PV, Schalij MJ, Srimahachota S, Jukema JW, Bruschke AV

Conventional stenting requires predilatation which potentially increases vessel wall injury and cost of the procedure. In this study, the safety and efficacy of direct Jostent Flex (Jomed AB, Helsingborg, Sweden) stent placement was evaluated in 50 patients. Quantitative coronary angiography was performed at baseline, post-stent and 6 months follow-up. Clinical follow-up was done up to 9 months. In 50 patients (38 male/12 female; age 61+/-12 years) with stable (n = 42; 84%) or unstable (n = 8; 1... Abstract

Cited 14 times since 2000 (0.6 per year) source: EuropePMC

Magnetic resonance imaging, Volume 18, Issue 3, 1 1 2000, Pages 245-253 Spin echo MRI in the evaluation of hearts with a double outlet right ventricle: usefulness and limitations. Beekmana RP, Roest AA, Helbing WA, Hazekamp MG, Schoof PH, Bartelings MM, Sobotka MA, de Roos A, Ottenkamp J

The surgical approach to a double outlet right ventricle (DORV) is dependent on the spatial relationship of the semilunar valves, outflow tracts and ventricular septal defect (VSD). The purpose of the study was to assess the value of MRI for the evaluation of cardiovascular anatomy in patients before and after surgery for DORV. Spin echo MRI was performed in 12 patients with DORV (eight preoperative and four postoperative patients). Thin-section MRI was performed in three orthogonal planes and s... Abstract

Cited 45 times since 2000 (1.8 per year) source: EuropePMC

Cardiovascular research, Volume 46, Issue 1, 1 1 2000, Pages 82-89 Comparison of intravenous and pulmonary artery injections of hypertonic saline for the assessment of conductance catheter parallel conductance. Steendijk P, Baan J

Objective: The conductance catheter provides a continuous measure of left ventricular volume. Conversion of raw data to calibrated absolute volume requires assessment of parallel conductance. Conventionally, parallel conductance is determined by injecting a small bolus hypertonic saline into the pulmonary artery and analyzing the signal obtained during passage of the bolus through the left ventricle. However, in some cases, a pulmonary artery catheter is not practicable. Therefore, we investigat... Abstract

Cited 33 times since 2000 (1.3 per year) source: EuropePMC

Virology, Volume 270, Issue 1, 1 1 2000, Pages 84-97 Genetic manipulation of equine arteritis virus using full-length cDNA clones: separation of overlapping genes and expression of a foreign epitope. de Vries AA, Glaser AL, Raamsman MJ, de Haan CA, Sarnataro S, Godeke GJ, Rottier PJ

Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae of the order Nidovirales. The unsegmented, infectious genome of EAV is 12,704 nt in length [exclusive of the poly(A) tail] and contains eight overlapping genes that are expressed from a 3'-coterminal nested set of seven leader-containing mRNAs. To investigate the importance of the overlapping gene arrangement in the viral life-cycle and to facilitate the genetic manipulation of th... Abstract

Cited 13 times since 2000 (0.5 per year) source: EuropePMC

European journal of nuclear medicine, Volume 27, Issue 3, 1 1 2000, Pages 302-307 Clinical evaluation of no-carrier-added meta-[123I]iodobenzylguanidine for myocardial scintigraphy. Knickmeier M, Matheja P, Wichter T, Schäfers KP, Kies P, Breithardt G, Schober O, Schäfers M

In clinical and research studies, images obtained using carrier-added meta-[123I]iodobenzylguanidine (c.a. [123I]MIBG) have shown quite variable quality, with varying levels of uptake in lung, liver and mediastinum; this is a significant problem for quantification of the myocardial uptake by means of region ratios. First experimental and preliminary human data in respect of no-carrier-added (n.c.a.) [123I]MIBG are indicative of improved imaging quality. The aim of the present study was to evalua... Abstract

Cited 44 times since 2000 (1.8 per year) source: EuropePMC

Arteriosclerosis, thrombosis, and vascular biology, Volume 20, Issue 3, 1 1 2000, Pages 744-750 Not acute but chronic hypertriglyceridemia is associated with impaired endothelium-dependent vasodilation: reversal after lipid-lowering therapy by atorvastatin. de Man FH, Weverling-Rijnsburger AW, van der Laarse A, Smelt AH, Jukema JW, Blauw GJ

There is controversy regarding the relation between hypertriglyceridemia (HTG) and endothelial function. This study was designed to investigate endothelial function in a patient group with chronic HTG, before and during lipid-lowering therapy by atorvastatin. In addition, the effects of acute HTG on endothelial function were studied in normolipidemic individuals. Eight male patients with chronic HTG were studied before and after 6 weeks of lipid-lowering treatment with 80 mg atorvastatin once da... Abstract

Cited 114 times since 2000 (4.6 per year) source: EuropePMC

Journal of virology, Volume 74, Issue 5, 1 1 2000, Pages 2333-2342 Characterization of the coronavirus mouse hepatitis virus strain A59 small membrane protein E. Raamsman MJ, Locker JK, de Hooge A, de Vries AA, Griffiths G, Vennema H, Rottier PJ

The small envelope (E) protein has recently been shown to play an essential role in the assembly of coronaviruses. Expression studies revealed that for formation of the viral envelope, actually only the E protein and the membrane (M) protein are required. Since little is known about this generally low-abundance virion component, we have characterized the E protein of mouse hepatitis virus strain A59 (MHV-A59), an 83-residue polypeptide. Using an antiserum to the hydrophilic carboxy terminus of t... Abstract

Cited 3 times since 2000 (0.1 per year) source: EuropePMC

Medicine and science in sports and exercise, Volume 32, Issue 3, 1 1 2000, Pages 571-575 Correlated neurocardiologic and fitness changes in athletes interrupting training. Frederiks J, Swenne CA, Bruschke AV, van der Velde ET, Maan AC, TenVoorde BJ, Vanrooijen MG, Mosterd WL, Schiereck P

Purpose: We studied nine male Dutch top marathon skaters during a 1-month interruption of their training schedules after their last contest in the winter to investigate a possible decline in baroreflex sensitivity. Methods: Before and after this period, a maximal exercise test was done, and at days 0, 4, 7, 14, and 28 neurocardiologic measurement sessions--heart rate and noninvasive baroreflex sensitivity, recumbent and tilt--were performed. Results: Interruption of training resulted in a signif... Abstract

Cited 26 times since 2000 (1 per year) source: EuropePMC

Congestive heart failure (Greenwich, Conn.), Volume 6, Issue 2, 1 1 2000, Pages 86-89 Bioimpedance monitoring: better than chest x-ray for predicting abnormal pulmonary fluid? Peacock WF, Albert NM, Kies P, White RD, Emerman CL

Bioimpedance monitoring may aid in treating heart failure. Mean thoracic electrical impedance (Zo) is inversely proportional to thoracic fluid volume and may offer greater sensitivity for detecting thoracic fluid. OBJECTIVE. Compare bioimpedance monitoring thoracic fluid detection to that of chest x-ray. METHOD. Prospective convenience sample. SETTING. 1000 bed teaching hospital. PARTICIPANTS. Patients with suspected heart failure and shortness of breath. A single blinded radiologist interpreted... Abstract

Cited 31 times since 2000 (1.2 per year) source: EuropePMC

Biochemistry, Volume 39, Issue 11, 1 1 2000, Pages 3023-3033 Detailed analysis of the phosphorylation of the human La (SS-B) autoantigen. (De)phosphorylation does not affect its subcellular distribution. Broekhuis CH, Neubauer G, van der Heijden A, Mann M, Proud CG, van Venrooij WJ, Pruijn GJ

The La (SS-B) autoantigen is an evolutionarily conserved phosphoprotein which plays an important role, most likely as an RNA chaperone, in various processes, such as the biosynthesis and maturation of RNA polymerase III transcripts in the cell nucleus and (internal) initiation of translation in the cytoplasm. In this study, the phosphorylation state of this protein from human HeLa and HEp-2 cells was characterized by high-resolution two-dimensional IEF/SDS-PAGE analysis, and phosphorylation site... Abstract