6173 results
Cited 32 times since 1995 (1.1 per year)
source: EuropePMC
Journal of virological methods,
Volume 54, Issue 1, 1 1 1995, Pages 1-13
Development and evaluation of an ELISA using recombinant fusion protein to detect the presence of host antibody to equine arteritis virus.
Chirnside ED, Francis PM, de Vries AA, Sinclair R, Mumford JA
A recombinant glutathione-S-transferase fusion protein expressing amino acids 55-98 of equine arteritis virus (EAV) GL (rGL 55-98) was tested in an ELISA for its ability to detect serum antibodies to EAV. Host antibodies induced following EAV infection bound the recombinant antigen by ELISA. The ELISA specificity and sensitivity were determined with a panel of equine sera including postinfection and postvaccination samples. A good correlation existed between EAV neutralizing antibody titers and...
Abstract
Cited 28 times since 1995 (0.9 per year)
source: EuropePMC
Journal of virology,
Volume 69, Issue 6, 1 1 1995, Pages 3441-3448
The small envelope glycoprotein (GS) of equine arteritis virus folds into three distinct monomers and a disulfide-linked dimer.
de Vries AA, Raamsman MJ, van Dijk HA, Horzinek MC, Rottier PJ
The small membrane glycoprotein (GS) of equine arteritis virus (EAV) is a minor virion component but is abundantly expressed in EAV-infected cells. In this study, we have analyzed its membrane topology, folding, oligomerization, and intracellular transport. We show that GS is a class I integral membrane protein with one functional N-glycosylation site. Gel electrophoresis under nonreducing conditions revealed that GS occurs in EAV-infected cells in four monomeric conformations and as disulfide-l...
Abstract
Cited 3 times since 1995 (0.1 per year)
source: EuropePMC
Archives of biochemistry and biophysics,
Volume 319, Issue 2, 1 1 1995, Pages 350-354
Metabolic inhibition of cardiomyocytes causes an increase in sarcolemmal fluidity which may be due to loss of cellular cholesterol.
Bastiaanse EM, Atsma DE, Van der Valk LJ, Van der Laarse A
We examined whether metabolic inhibition (5 mM NaCN + 10 mM 2-deoxyglucose) affects sarcolemmal fluidity in cultured neonatal cardiomyocytes. As a measure of sarcolemmal fluidity we determined the fluorescence steady-state anisotropy (rss, which is reciprocally related to membrane fluidity) of cardiomyocytes labeled with 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene, p-toluenesulfonate. During metabolic inhibition, membrane fluidity increased progressively: after 30 min rss had fallen...
Abstract
Cited 57 times since 1995 (1.9 per year)
source: EuropePMC
Circulation research,
Volume 76, Issue 6, 1 1 1995, Pages 1071-1078
Role of calcium-activated neutral protease (calpain) in cell death in cultured neonatal rat cardiomyocytes during metabolic inhibition.
Atsma DE, Bastiaanse EM, Jerzewski A, Van der Valk LJ, Van der Laarse A
Calcium-activated neutral protease (CANP), also known as calpain, has been implicated in the development of cell death in ischemic hearts. CANP is thought to be activated by the calcium overload that develops during ischemia. We studied the involvement of CANP in cell death in cultured neonatal rat cardiomyocytes during metabolic inhibition (5 mmol/L NaCN + 10 mmol/L 2-deoxyglucose). First, we isolated CANP using ion exchange and affinity chromatography. Then the efficacy of the CANP inhibitors...
Abstract
Cited 426 times since 1995 (14.2 per year)
source: EuropePMC
Circulation,
Volume 91, Issue 10, 1 1 1995, Pages 2528-2540
Effects of lipid lowering by pravastatin on progression and regression of coronary artery disease in symptomatic men with normal to moderately elevated serum cholesterol levels. The Regression Growth Evaluation Statin Study (REGRESS).
Jukema JW, Bruschke AV, van Boven AJ, Reiber JH, Bal ET, Zwinderman AH, Jansen H, Boerma GJ, van Rappard FM, Lie KI
Background: Intensive lowering of serum cholesterol may retard progression of coronary atherosclerosis in selected groups of patients. However, few data are available on the potential benefit of serum cholesterol reduction in the broad range of patients with coronary atherosclerosis and normal to moderately elevated serum cholesterol levels who undergo various forms of treatment. The Regression Growth Evaluation Statin Study (REGRESS) addresses this group of patients. Methods and results: REGRES...
Abstract
Cited 19 times since 1995 (0.6 per year)
source: EuropePMC
Journal of the American College of Cardiology,
Volume 25, Issue 6, 1 1 1995, Pages 1428-1435
Interaction between afterload and contractility in the newborn heart: evidence of homeometric autoregulation in the intact circulation.
Klautz RJ, Teitel DF, Steendijk P, van Bel F, Baan J
Objectives: We undertook the present study to determine whether afterload and contractility interact in the hearts of newborn lambs. We specifically investigated whether stepwise increases in afterload increase contractility. Background: Several studies in the isolated and intact adult dog heart have shown that afterload and contractility are not independent determinants of cardiac performance; rather, they interact. Afterload and contractility are unlikely to interact in the newborn heart becau...
Abstract
Cited 2 times since 1995 (0.1 per year)
source: EuropePMC
Circulation,
Volume 91, Issue 8, 1 1 1995, Pages 2292-2293
Sympathovagal balance and graded orthostatic tilt.
Swenne CA, Bootsma M
Abstract
Cited 3 times since 1995 (0.1 per year)
source: EuropePMC
American heart journal,
Volume 129, Issue 4, 1 1 1995, Pages 809-818
Magnetic resonance techniques for the assessment of myocardial viability: clinical experience.
Vliegen HW, de Roos A, Bruschke AV, van der Wall EE
Abstract
Cited 51 times since 1995 (1.7 per year)
source: EuropePMC
Circulation,
Volume 91, Issue 7, 1 1 1995, Pages 2010-2017
Beat-to-beat analysis of left ventricular pressure-volume relation and stroke volume by conductance catheter and aortic Modelflow in cardiomyoplasty patients.
Schreuder JJ, van der Veen FH, van der Velde ET, Delahaye F, Alfieri O, Jegaden O, Lorusso R, Jansen JR, van Ommen V, Finet G
Background: Since the clinical introduction of dynamic cardiomyoplasty, a discrepancy has been observed between unchanged measurements of cardiac function and improved clinical outcome. Methods and results: We performed a beat-to-beat analysis of cardiac performance at rest in nine cardiomyoplasty patients 6 to 24 months after operation. Conductance and micromanometer catheters were placed in left ventricle and aorta and used for measurements over a 15-second period, during which the wrapped lat...
Abstract
Cited 4 times since 1995 (0.1 per year)
source: EuropePMC
The Journal of investigative dermatology,
Volume 104, Issue 3, 1 1 1995, Pages 374-378
Differential regulation of plasminogen activation in normal keratinocytes and SCC-4 cells by fibroblasts.
Boxman IL, Quax PH, Löwik CW, Papapoulos SE, Verheijen J, Ponec M
The plasminogen activator (PA)/plasmin system is thought to be involved in processes such as tumor invasion and wound healing, during which epithelial and mesenchymal cells come close together. However, information on regulation of the PA/plasmin system during epithelial-mesenchymal interactions is scarce. Therefore, we examined the in vitro modulation of the production and activity of the components of the PA/plasmin system in squamous carcinoma cells (SCC-4) and normal human keratinocytes in r...
Abstract
Cited 62 times since 1995 (2.1 per year)
source: EuropePMC
Virology,
Volume 207, Issue 2, 1 1 1995, Pages 518-527
Identification of a neutralization site in the major envelope glycoprotein (GL) of equine arteritis virus.
Balasuriya UB, Maclachlan NJ, De Vries AA, Rossitto PV, Rottier PJ
A panel of six neutralizing monoclonal antibodies (MAbs), neutralization-resistant variant (escape mutant [EM]) viruses, and individual viral proteins derived from a vaccinia virus expression system were used to identify the neutralizing determinants of equine arteritis virus (EAV). The neutralizing MAbs recognize a single neutralization site on the 29-kDa envelope glycoprotein of EAV (U. B. R. Balasuriya et al., 1993, J. Gen. Virol., 74, 2525-2529). Vaccinia virus recombinants which express eit...
Abstract
Cited 128 times since 1995 (4.2 per year)
source: EuropePMC
Circulation research,
Volume 76, Issue 2, 1 1 1995, Pages 199-208
cGMP and nitric oxide modulate thrombin-induced endothelial permeability. Regulation via different pathways in human aortic and umbilical vein endothelial cells.
Draijer R, Atsma DE, van der Laarse A, van Hinsbergh VW
Previous studies have demonstrated that cGMP and cAMP reduce the endothelial permeability for fluids and macromolecules when the endothelial permeability is increased by thrombin. In this study, we have investigated the mechanism by which cGMP improves the endothelial barrier function and examined whether nitric oxide (NO) can serve as an endogenous modulator of endothelial barrier function. Thrombin increased the passage of macromolecules through human umbilical vein and human aortic endothelia...
Abstract
Cited 11 times since 1995 (0.4 per year)
source: EuropePMC
Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer,
Volume 139, 1 1 1995, Pages 105-122
Properties of metastasizing and nonmetastasizing human melanoma cells.
van Muijen GN, Danen EH, de Vries TJ, Quax PH, Verheijen JH, Ruiter DJ
Dissemination of tumor cells includes several steps, such as: (a) detachment of tumor cells from the primary tumor, (b) traversement of the basement membrane, and (c) migration into the extracellular matrix. In these processes, at least two important categories of proteins are involved: proteases and adhesion molecules. In this contribution we describe the expression and function of components of the plasminogen activator (PA) system (proteases) and of integrins (cell-matrix adhesion molecules)...
Abstract
Cited 22 times since 1995 (0.7 per year)
source: EuropePMC
The American journal of cardiovascular pathology,
Volume 5, Issue 1, 1 1 1995, Pages 27-31
Polyploidy of myocyte nuclei in pressure overloaded human hearts: a flow cytometric study in left and right ventricular myocardium.
Vliegen HW, Eulderink F, Bruschke AV, van der Laarse A, Cornelisse CJ
Increased heart weight is known to be associated with polyploidy of myocardial nuclei. However, the relation between polyploidy and myocardial hypertrophy at a cellular level, i.e. myocyte hypertrophy, has not yet been investigated. The present study was conducted to reveal this relation in different regions in the heart using two recently developed techniques, i.e. flow cytometry to measure polyploidy and microscopic morphometry to measure myocyte dimensions in fixed tissue sections. We investi...
Abstract
Cited 21 times since 1995 (0.7 per year)
source: EuropePMC
The Journal of pathology,
Volume 175, Issue 1, 1 1 1995, Pages 59-67
Components of the plasminogen activation system in uveal melanoma--a clinico-pathological study.
De Vries TJ, Mooy CM, Van Balken MR, Luyten GP, Quax PH, Verspaget HW, Weidle UH, Ruiter DJ, Van Muijen GN
In tumour development, proteases such as plasminogen activators (PAs) play a role in degradation of the extracellular matrix and other tissue barriers. Recently, we demonstrated that plasminogen activators, their inhibitors, and urokinase receptor emerge in late stages of cutaneous melanocytic tumour progression. In this study we investigated the expression and distribution of the various components of the PA system and the presence of PA enzyme activity in 45 freshly frozen primary uveal melano...
Abstract
Nederlands tijdschrift voor geneeskunde,
Volume 138, Issue 50, 1 1 1994, Pages 2502-2506
[Nitric oxide to combat pulmonary hypertension in an infant following heart surgery for septum defect].
Gesink-van der Veer BJ, Hazekamp MG, de Beaufort AJ, Stolk B, Helbing WA
A 5-month-old female infant with Down syndrome underwent surgical repair of a complete atrioventricular septal defect and a patent ductus arteriosus. The postoperative course was complicated by life-threatening pulmonary hypertensive crises which did not respond to conventional vasodilating therapy. Nitric oxide inhalation resulted in rapid decrease in pulmonary artery pressure and improvement of haemodynamics and oxygenation without causing any untoward side effects. Inhalation of NO was contin...
Abstract
Cited 43 times since 1994 (1.4 per year)
source: EuropePMC
Gastroenterology,
Volume 107, Issue 5, 1 1 1994, Pages 1449-1456
Inactive urokinase and increased levels of its inhibitor type 1 in colorectal cancer liver metastasis.
Sier CF, Vloedgraven HJ, Ganesh S, Griffioen G, Quax PH, Verheijen JH, Dooijewaard G, Welvaart K, van de Velde CJ, Lamers CB
Background/aims: Human colorectal carcinogenesis was previously found to be associated with an increased urokinase-type plasminogen activator expression, both in antigen and activity, accompanied by simultaneously enhanced levels of plasminogen activator inhibitors type 1 and type 2. This increased proteolytic activity may contribute to invasive growth and metastasis of the tumors. Methods: In the present study, homogenates of liver metastases, primary colorectal carcinomas, and adjacent normal...
Abstract
Cited 3 times since 1994 (0.1 per year)
source: EuropePMC
Surgical endoscopy,
Volume 8, Issue 11, 1 1 1994, Pages 1335-1337
Liver fibrosis after extracorporeal shock-wave lithotripsy of gallbladder stones. A case report.
Plaisier PW, Hamming JF, van der Hul RL, den Toom R, Bruining HA
We encountered significant liver fibrosis in a healthy young patient undergoing laparoscopic cholecystectomy for symptomatic gallstone disease. Twelve months prior to cholecystectomy the patient underwent multiple extracorporeal shock-wave lithotripsy (ESWL) sessions with adjuvant oral bile-acid therapy. Since the site of fibrosis corresponded clearly to the shock-wave transmission path, which was in accordance with animal studies, it was concluded that this liver fibrosis was a side effect of b...
Abstract
Cited 2 times since 1994 (0.1 per year)
source: EuropePMC
The American journal of physiology,
Volume 267, Issue 5 Pt 2, 1 1 1994, Pages H1895-906
Determinants of end-systolic pressure during different load alterations in the in situ left ventricle.
van der Linden LP, van der Velde ET, van Houwelingen HC, Bruschke AV, Baan J
Because of the strong dependency of the end-systolic pressure-volume relation on the type of transient loading intervention in the in situ left ventricle (LV), experiments in the basal inotropic state in 16 open-chest anesthetized dogs were reanalyzed to find additional variables to model and predict end-systolic pressure (ESP) of both afterloading and preloading interventions by a single equation. Random-coefficients regression analysis was performed on 22 experiments in the basal inotropic sta...
Abstract
Cited 1 times since 1994 (0 per year)
source: EuropePMC
The British journal of nutrition,
Volume 72, Issue 4, 1 1 1994, Pages 567-577
Dietary fructose v. glucose in rats raises urinary excretion, true absorption and ileal solubility of magnesium but decreases magnesium retention.
van der Heijden A, van den Berg GJ, Lemmens AG, Beynen AC
Apparent Mg absorption, that is Mg intake minus faecal excretion, was found to be greater in rats fed on diets containing fructose instead of glucose. This effect of fructose was accompanied by enhanced urinary excretion and diminished retention of Mg. True Mg absorption was then determined with the use of oral and intraperitoneal administration of tracer doses of 28 Mg. True Mg absorption was significantly greater in rats fed on fructose. There was no significant effect of fructose v. glucose o...
Abstract
