Research

Publications

Below you can find a list of our published research.

8892 results

Cited 16 times since 2013 (1.3 per year) source: EuropePMC

PloS one, Volume 8, Issue 3, 6 1 2013, Pages e58572 Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro. de Graaf M, Herfst S, Aarbiou J, Burgers PC, Zaaraoui-Boutahar F, Bijl M, van Ijcken W, Schrauwen EJ, Osterhaus AD, Luider TM, Scholte BJ, Fouchier RA, Andeweg AC

Human metapneumovirus (HMPV) encodes a small hydrophobic (SH) protein of unknown function. HMPV from which the SH open reading frame was deleted (HMPVΔSH) was viable and displayed similar replication kinetics, cytopathic effect and plaque size compared with wild type HMPV in several cell-lines. In addition, no differences were observed in infection efficiency or cell-to-cell spreading in human primary bronchial epithelial cells (HPBEC) cultured at an air-liquid interphase. Host gene expression w... Abstract

Cited 5 times since 2013 (0.4 per year) source: EuropePMC

Heart (British Cardiac Society), Volume 99, Issue 8, 6 1 2013, Pages 556-561 Association between discharge heart rate and left ventricular adverse remodelling in ST segment elevation myocardial infarction patients treated with primary percutaneous coronary intervention. Joyce E, Hoogslag GE, Leong DP, Fox K, Schalij MJ, Marsan NA, Bax JJ, Delgado V

Objective: Left ventricular (LV) adverse remodelling is an important determinant of mortality after ST segment elevation myocardial infarction (STEMI). Recently, discharge heart rate (DHR) has been associated with long-term outcomes after STEMI. Whether DHR is related to the development of LV remodelling after STEMI remains unknown. The present study evaluated the association between DHR after STEMI and the occurrence of LV remodelling at 6 months. DESIGN, SETTING, PATIENTS AND INTERVENTIONS: In... Abstract

Cited 25 times since 2013 (2 per year) source: EuropePMC

The pharmacogenomics journal, Volume 14, Issue 1, 5 1 2013, Pages 6-13 Drug-gene interactions and the search for missing heritability: a cross-sectional pharmacogenomics study of the QT interval. Avery CL, Sitlani CM, Arking DE, Arnett DK, Bis JC, Boerwinkle E, Buckley BM, Ida Chen YD, de Craen AJ, Eijgelsheim M, Enquobahrie D, Evans DS, Ford I, Garcia ME, Gudnason V, Harris TB, Heckbert SR, Hochner H, Hofman A, Hsueh WC, Isaacs A, Jukema JW, Knekt P, Kors JA, Krijthe BP, Kristiansson K, Laaksonen M, Liu Y, Li X, Macfarlane PW, Newton-Cheh C, Nieminen MS, Oostra BA, Peloso GM, Porthan K, Rice K, Rivadeneira FF, Rotter JI, Salomaa V, Sattar N, Siscovick DS, Slagboom PE, Smith AV, Sotoodehnia N, Stott DJ, Stricker BH, Stürmer T, Trompet S, Uitterlinden AG, van Duijn C, Westendorp RG, Witteman JC, Whitsel EA, Psaty BM

Variability in response to drug use is common and heritable, suggesting that genome-wide pharmacogenomics studies may help explain the 'missing heritability' of complex traits. Here, we describe four independent analyses in 33 781 participants of European ancestry from 10 cohorts that were designed to identify genetic variants modifying the effects of drugs on QT interval duration (QT). Each analysis cross-sectionally examined four therapeutic classes: thiazide diuretics (prevalence of... Abstract

Cited 150 times since 2013 (11.9 per year) source: EuropePMC

Annals of neurology, Volume 73, Issue 3, 4 1 2013, Pages 397-407 Autoantibodies to cytosolic 5'-nucleotidase 1A in inclusion body myositis. Pluk H, van Hoeve BJ, van Dooren SH, Stammen-Vogelzangs J, van der Heijden A, Schelhaas HJ, Verbeek MM, Badrising UA, Arnardottir S, Gheorghe K, Lundberg IE, Boelens WC, van Engelen BG, Pruijn GJ

Objective: Sporadic inclusion body myositis (sIBM) is an inflammatory myopathy characterized by both degenerative and autoimmune features. In contrast to other inflammatory myopathies, myositis-specific autoantibodies had not been found in sIBM patients until recently. We used human skeletal muscle extracts as a source of antigens to detect autoantibodies in sIBM and to characterize the corresponding antigen. Methods: Autoantibodies to skeletal muscle antigens were detected by immunoblotting. Th... Abstract

Cited 205 times since 2013 (16.2 per year) source: EuropePMC

Circulation, Volume 127, Issue 14, 4 1 2013, Pages 1466-75, 1475e1-28 MicroRNA29: a mechanistic contributor and potential biomarker in atrial fibrillation. Dawson K, Wakili R, Ordög B, Clauss S, Chen Y, Iwasaki Y, Voigt N, Qi XY, Sinner MF, Dobrev D, Kääb S, Nattel S

Background: Congestive heart failure (CHF) causes atrial fibrotic remodeling, a substrate for atrial fibrillation (AF) maintenance. MicroRNA29 (miR29) targets extracellular matrix proteins. In the present study, we examined miR29b changes in patients with AF and/or CHF and in a CHF-related AF animal model and assessed its potential role in controlling atrial fibrous tissue production. Methods and results: Control dogs were compared with dogs subjected to ventricular tachypacing for 24 hours, 1 w... Abstract

Cited 22 times since 2013 (1.7 per year) source: EuropePMC

Netherlands heart journal : monthly journal of the Netherlands Society of Cardiology and the Netherlands Heart Foundation, Volume 21, Issue 3, 1 1 2013, Pages 113-117 Ebstein's anomaly may be caused by mutations in the sarcomere protein gene MYH7. van Engelen K, Postma AV, van de Meerakker JB, Roos-Hesselink JW, Helderman-van den Enden AT, Vliegen HW, Rahman T, Baars MJ, Sels JW, Bauer U, Pickardt T, Sperling SR, Moorman AF, Keavney B, Goodship J, Klaassen S, Mulder BJ

Ebstein's anomaly is a rare congenital heart malformation characterised by adherence of the septal and posterior leaflets of the tricuspid valve to the underlying myocardium. Associated abnormalities of left ventricular morphology and function including left ventricular noncompaction (LVNC) have been observed. An association between Ebstein's anomaly with LVNC and mutations in the sarcomeric protein gene MYH7, encoding β-myosin heavy chain, has been shown by recent studies. This might... Abstract

Cited 31 times since 2013 (2.5 per year) source: EuropePMC

Pharmacogenetics and genomics, Volume 23, Issue 3, 1 1 2013, Pages 148-155 Impact of POR*28 on the clinical pharmacokinetics of CYP3A phenotyping probes midazolam and erythromycin. Elens L, Nieuweboer AJ, Clarke SJ, Charles KA, de Graan AJ, Haufroid V, van Gelder T, Mathijssen RH, van Schaik RH

Objective: P450 oxidoreductase (POR) is essential for cytochrome P450 (CYP) activity in humans. The POR*28 allele (A503V) has been shown to impact on in-vitro CYP-mediated metabolism, including CYP3A isoenzymes. The aim of the present study was to determine the in vivo impact of the POR*28 allele on the pharmacokinetics of the classic CYP3A phenotyping probes midazolam and erythromycin. Whereas midazolam is metabolized by both CYP3A4 and CYP3A5, erythromycin is exclusively oxidized by CYP3A4. Ma... Abstract

Cited 11 times since 2013 (0.9 per year) source: EuropePMC

Circulation, Volume 127, Issue 12, 1 1 2013, Pages 1340-1341 Compression of the left main coronary artery by a giant pulmonary artery aneurysm. Jurado-Román A, Hernández-Hernández F, Ruíz-Cano MJ, Velázquez-Martín MT, Medina JM, Pérez-López I, Barrios-Garrido-Lestache E, Montero-Cabezas JM, Escribano-Subías P

Abstract

Cited 7 times since 2013 (0.6 per year) source: EuropePMC

Annals of plastic surgery, Volume 70, Issue 3, 1 1 2013, Pages 360-365 Intraoperative prediction of postoperative flap outcome using the near-infrared fluorophore methylene blue. Ashitate Y, Lee BT, Laurence RG, Lunsford E, Hutteman M, Oketokoun R, Choi HS, Frangioni JV

Methylene blue (MB) is a near-infrared fluorophore that provides a stable visual map of skin perfusion after intravenous injection. We explored the capability of MB to predict submental flap postoperative outcome using a single intraoperative measurement. Submental flaps were created in N = 15 pigs and imaged using the FLARE imaging system immediately after surgery and at 72 hours. Using the first 3 pigs, optimal MB dosing was found to be 2.0 mg/kg. Training and validation sets of 6 pigs each we... Abstract

Cited 94 times since 2013 (7.4 per year) source: EuropePMC

The Journal of urology, Volume 190, Issue 2, 1 1 2013, Pages 574-579 Intraoperative near infrared fluorescence guided identification of the ureters using low dose methylene blue: a first in human experience. Verbeek FP, van der Vorst JR, Schaafsma BE, Swijnenburg RJ, Gaarenstroom KN, Elzevier HW, van de Velde CJ, Frangioni JV, Vahrmeijer AL

Purpose: Near infrared fluorescence imaging is a promising technique that offers real-time visual information during surgery. In this study we report the first clinical results to our knowledge of ureteral imaging using near infrared fluorescence after a simple peripheral infusion of methylene blue. Furthermore, we assessed the optimal timing and dose of methylene blue. Materials and methods: A total of 12 patients who underwent lower abdominal surgery were included in this prospective feasibili... Abstract

Cited 57 times since 2013 (4.5 per year) source: EuropePMC

PLoS genetics, Volume 9, Issue 2, 28 4 2013, Pages e1003301 Deleterious alleles in the human genome are on average younger than neutral alleles of the same frequency. Kiezun A, Pulit SL, Francioli LC, van Dijk F, Swertz M, Boomsma DI, van Duijn CM, Slagboom PE, van Ommen GJ, Wijmenga C, Genome of the Netherlands Consortium, de Bakker PI, Sunyaev SR

Large-scale population sequencing studies provide a complete picture of human genetic variation within the studied populations. A key challenge is to identify, among the myriad alleles, those variants that have an effect on molecular function, phenotypes, and reproductive fitness. Most non-neutral variation consists of deleterious alleles segregating at low population frequency due to incessant mutation. To date, studies characterizing selection against deleterious alleles have been based on all... Abstract

Cited 69 times since 2013 (5.5 per year) source: EuropePMC

The Journal of infectious diseases, Volume 207, Issue 11, 27 4 2013, Pages 1780-1786 Identification of a genetic determinant in clinical Enterococcus faecium strains that contributes to intestinal colonization during antibiotic treatment. Zhang X, Top J, de Been M, Bierschenk D, Rogers M, Leendertse M, Bonten MJ, van der Poll T, Willems RJ, van Schaik W

Intestinal colonization by antibiotic-resistant Enterococcus faecium is the first step in a process that can lead to infections in hospitalized patients. By comparative genome analysis and subsequent polymerase chain reaction screening, we identified a locus that encodes a putative phosphotransferase system (PTS). The PTS locus was widespread in isolates from hospital outbreaks of infection (84.2%) and nonoutbreak clinical infections (66.0%) but absent from human commensal isolates. Deletion of... Abstract

Cited 5 times since 2013 (0.4 per year) source: EuropePMC

EJIFCC, Volume 24, Issue 3, 21 3 2013, Pages 105-112 Clinical Application of Pharmacogenetics: Where are We Now? van Schaik RH, IFCC Task Force on Pharmacogenetics: Prof Dr Maurizio Ferrari (IT), Prof Dr Michael Neumaier (GER), Prof Dr Munir Pirmohamed (UK), Prof Dr Henk-Jan Guchelaar (NL), Prof Dr Ron van Schaik (NL)

Pharmacogenetic (PGx) testing has the potential to improve drug therapy in an individual by informing appropriate drug dosing or drug selection in order to maximize efficacy and safety. Although multiple studies have illustrated the potential benefits of such testing when applied to specific drugs across a broad range of therapy areas, the uptake of PGx testing in routine clinical practice has been relatively limited. Implementation appears to be hampered by the absence of sufficiently strong ev... Abstract

Cited 16 times since 2013 (1.3 per year) source: EuropePMC

PLoS computational biology, Volume 9, Issue 2, 21 3 2013, Pages e1002874 Cost and effects of different admission screening strategies to control the spread of methicillin-resistant Staphylococcus aureus. Gurieva T, Bootsma MC, Bonten MJ

Nosocomial infection rates due to antibiotic-resistant bacteriae, e.g., methicillin-resistant Staphylococcus aureus (MRSA) remain high in most countries. Screening for MRSA carriage followed by barrier precautions for documented carriers (so-called screen and isolate (S&I)) has been successful in some, but not all settings. Moreover, different strategies have been proposed, but comparative studies determining their relative effects and costs are not available. We, therefore, used a mathemati... Abstract

Cited 42 times since 2013 (3.3 per year) source: EuropePMC

BJOG : an international journal of obstetrics and gynaecology, Volume 120, Issue 6, 19 3 2013, Pages 758-764 Near-infrared fluorescence sentinel lymph node biopsy in vulvar cancer: a randomised comparison of lymphatic tracers. Schaafsma BE, Verbeek FP, Peters AA, van der Vorst JR, de Kroon CD, van Poelgeest MI, Trimbos JB, van de Velde CJ, Frangioni JV, Vahrmeijer AL, Gaarenstroom KN

This study aims to confirm the feasibility of near-infrared (NIR) fluorescence imaging for sentinel lymph node (SLN) biopsy in vulvar cancer and to compare the tracer indocyanine green (ICG) bound to human serum albumin (HSA) versus ICG alone. Women received 99mTc-nanocolloid and patent blue for SLN detection. Subsequently, women randomly received ICG:HSA or ICG alone. In 24 women, 35 SLNs were intraoperatively detected. All SLNs detected were radioactive and NIR fluorescent and 27 (77%) were bl... Abstract

Cited 28 times since 2013 (2.2 per year) source: EuropePMC

Circulation. Arrhythmia and electrophysiology, Volume 6, Issue 2, 18 3 2013, Pages 380-391 Engraftment patterns of human adult mesenchymal stem cells expose electrotonic and paracrine proarrhythmic mechanisms in myocardial cell cultures. Askar SF, Ramkisoensing AA, Atsma DE, Schalij MJ, de Vries AA, Pijnappels DA

Background: After intramyocardial injection, mesenchymal stem cells (MSCs) may engraft and influence host myocardium. However, engraftment rate and pattern of distribution are difficult to control in vivo, hampering assessment of potential adverse effects. In this study, the role of the engraftment patterns of MSCs on arrhythmicity in controllable in vitro models is investigated. Methods and results: Cocultures of 4×10(5) neonatal rat cardiomyocytes and 7% or 28% adult human MSCs (hMSCs) in diff... Abstract

Cited 152 times since 2013 (12 per year) source: EuropePMC

The international journal of cardiovascular imaging, Volume 29, Issue 5, 16 3 2013, Pages 1177-1190 Automatic quantification and characterization of coronary atherosclerosis with computed tomography coronary angiography: cross-correlation with intravascular ultrasound virtual histology. de Graaf MA, Broersen A, Kitslaar PH, Roos CJ, Dijkstra J, Lelieveldt BP, Jukema JW, Schalij MJ, Delgado V, Bax JJ, Reiber JH, Scholte AJ

Plaque constitution on computed tomography coronary angiography (CTA) is associated with prognosis. At present only visual assessment of plaque constitution is possible. An accurate automatic, quantitative approach for CTA plaque constitution assessment would improve reproducibility and allows higher accuracy. The present study assessed the feasibility of a fully automatic and quantitative analysis of atherosclerosis on CTA. Clinically derived CTA and intravascular ultrasound virtual histology (... Abstract

Cited 14 times since 2013 (1.1 per year) source: EuropePMC

PloS one, Volume 8, Issue 2, 15 3 2013, Pages e56262 Non-homologous end-joining pathway associated with occurrence of myocardial infarction: gene set analysis of genome-wide association study data. Verschuren JJ, Trompet S, Deelen J, Stott DJ, Sattar N, Buckley BM, Ford I, Heijmans BT, Guchelaar HJ, Houwing-Duistermaat JJ, Slagboom PE, Jukema JW

Purpose: DNA repair deficiencies have been postulated to play a role in the development and progression of cardiovascular disease (CVD). The hypothesis is that DNA damage accumulating with age may induce cell death, which promotes formation of unstable plaques. Defects in DNA repair mechanisms may therefore increase the risk of CVD events. We examined whether the joints effect of common genetic variants in 5 DNA repair pathways may influence the risk of CVD events. Methods: The PLINK set-based t... Abstract

Cited 16 times since 2013 (1.3 per year) source: EuropePMC

Systematic reviews, Volume 2, 15 3 2013, Pages 13 Individual patient data meta-analysis for the clinical assessment of coronary computed tomography angiography: protocol of the Collaborative Meta-Analysis of Cardiac CT (CoMe-CCT). Schuetz GM, Schlattmann P, Achenbach S, Budoff M, Garcia MJ, Roehle R, Pontone G, Meijboom WB, Andreini D, Alkadhi H, Honoris L, Bettencourt N, Hausleiter J, Leschka S, Gerber BL, Meijs MF, Shabestari AA, Sato A, Zimmermann E, Schoepf UJ, Diederichsen A, Halon DA, Mendoza-Rodriguez V, Hamdan A, Nørgaard BL, Brodoefel H, Ovrehus KA, Jenkins SM, Halvorsen BA, Rixe J, Sheikh M, Langer C, Martuscelli E, Romagnoli A, Scholte AJ, Marcus RP, Ulimoen GR, Nieman K, Mickley H, Nikolaou K, Tardif JC, Johnson TR, Muraglia S, Chow BJ, Maintz D, Laule M, Dewey M

Unlabelled: Background: Coronary computed tomography angiography has become the foremost noninvasive imaging modality of the coronary arteries and is used as an alternative to the reference standard, conventional coronary angiography, for direct visualization and detection of coronary artery stenoses in patients with suspected coronary artery disease. Nevertheless, there is considerable debate regarding the optimal target population to maximize clinical performance and patient benefit. The most... Abstract

Cited 24 times since 2013 (1.9 per year) source: EuropePMC

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, Volume 44, Issue 2, 13 2 2013, Pages 269-274 Disparity in right vs left ventricular recovery during follow-up after ventricular septal defect correction in children. Klitsie LM, Kuipers IM, Roest AA, Van der Hulst AE, Stijnen T, Hazekamp MG, Blom NA, Ten Harkel AD

Objectives: Long-term prognosis after ventricular septal defect (VSD) correction in childhood is excellent. Nevertheless, decreased biventricular systolic performance has been described immediately following VSD surgery in children. In an effort to better understand this decrease and its time-course, we characterized biventricular systolic performance following VSD closure in paediatric patients up to 20 months postoperatively. Methods: Thirty-nine children undergoing VSD surgery and 22 age-matc... Abstract