Cited 16 times since 2013 (1.3 per year) source: EuropePMC Bioorganic & medicinal chemistry letters, Volume 23, Issue 13, 9 2 2013, Pages 3749-3752 Improving the biological activity of the antimicrobial peptide anoplin by membrane anchoring through a lipophilic amino acid derivative. Slootweg JC, van Schaik TB, Quarles van Ufford HL, Breukink E, Liskamp RM, Rijkers DT
The lipophilic amino acid, (S)-2-aminoundecanoic acid, was synthesized and incorporated at a number of specific positions within the peptide sequence of anoplin. These lipophilic anoplin analogs showed to be more active against Escherichia coli and Staphylococcus aureus compared to native anoplin, while the EC50-value of hemolysis was at least one order of magnitude lower than the MIC values. This was in sharp contrast to the N-acylated anoplin derivative, where a gain in activity also led to a complete loss of selectivity. Thus, the incorporation of a lipophilic amino acid residue into anoplin enhanced the antimicrobial activity, while selectivity towards microbial membranes was retained.
